This checklist dataset encompasses Arachnofauna species observed during the 'objective 1000' project, in which the entomofauna of the Jean Massart Botanical Garden was studied. First entomological prospectings started in 2013-2014 led by the Royal Belgian Institute of Natural Sciences (RBINS). The richness of the species led to a collective enthusiasm that allowed the implementation of the 'objective 1000' project with the collaboration of Brussels Environment (2015-2021). Please feel free to get in touch with us if you have any question or remark regarding the objective 1000 project or this dataset content.