The “checklist of alien seed plants of D.R. Congo, based on evidence from herbarium collections” is a species checklist dataset published by the Botanic Garden Meise. It contains information on 436 alien plant species occurring in the Democratic Republic of the Congo, based on evidence from herbarium collections (Bordar & Meerts 2022). The plant species were registered between 1869 and 2017. Here it is published as a standardized Darwin Core Archive and includes for each species: the scientific name, kingdom, family and stable taxon identifier (in the taxon core), the year of first introduction and last assessment in Congo and the degree of establishment (in the distribution extension) and the native range and life form (in the description extension). Issues with the dataset can be reported at https://github.com/alien-csi/alien-flora-congo-checklist/issues. We have released this dataset to the public domain under a Creative Commons Zero waiver. We would appreciate it if you follow the INBO norms for data use (https://www.inbo.be/en/norms-data-use) when using the data. If you have any questions regarding this dataset, don't hesitate to contact us via the contact information provided in the metadata or via opendata@inbo.be.